Hydrophilic interaction chromatography (HILIC) is a popular technique to analyze very polar molecules. However, it is often difficult to develop a robust method.
HILIC Columns: Disadvantages
・ Extremely sensitive to sample solvent used resulting in shifting retention time.
・ Small sample loading capacity – polar molecules do not dissolve well in organic solvents.
・ Peak distortion occurs if sample solvents are different than the mobile phase.
We launch a new product line in HPLC column category based on the core-shell technology, which help customers build method development up. The kit contains three reversed phase columns you can expect different selectivities and is economical - the same price as two columns. http://www.nacalaiusa.com/products/view/326/cosmocore-method-development-kit
We attend the SFC Day San Diego which will bring together leading separation scientists and engineers from major pharmaceutical and chemical companies, academic labs and government agencies.
- Learn the latest innovations in this burgeoning technology by participating in the meeting events including: Lectures by chromatography experts sharing case studies and new developments
- Poster sessions offering personalized explanations of lab experiments and results
- Exhibits of cutting-edge technology
- Vendor workshops providing training
- Social gatherings to network http://www.greenchemistrygroup.org/#!san-diego-registration/hhhn6
Please stop by our stand, #1155, to see the latest products.
- Cardiomyocyte Differentiation Medium: Production of high quality cardiomyocytes
- Xeno-free recombinant peptide has an increased arginine-glycine-aspartate (RGD) sequences with high cellular adhesion.
- EZSPHERE® has a lot of evenly designed micro wells on the surface. Inoculated cells drop into the micro wells and form uniformly sized Spheroids.
Feb. 22nd Monday 7:00 PM Poster # 53
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Topic: Metabolomics
Separating Vitamin D2 D3, their 25-OH Metabolites and C-3 Epimers
The accuracy of current vitamin D measurements by immunoassays and LCMS has been questioned due to the overlapping LC peaks with identical m/z values epimers and isobars. To solve this problem, we have developed a new HPLC method to achieve baseline separation of vitamin D2/D3, their 25-OH metabolites and C3-epimers in one single run.
Download (PDF) http://www.nacalai.co.jp/global/cosmosil/pdf/MSACL2016_vitaminD.pdf
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Feb. 22 nd Monday 7:00 PM Poster # 54
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Topic: Small Molecule Analytes
Detecting THC Metabolites and Other Cannabinoids
In the first part, Δ9-THC, 11-hydroxy-Δ9-THC and 11-nor-9-carboxy-Δ9-THC are detected using a simple gradient. In the second part, four cannabinoids, CBD, CBN, Δ9-THC, and Δ8-THC were baseline separated making quantification easy.
Download (PDF) http://www.nacalai.co.jp/global/cosmosil/pdf/MSACL2016_cannabinoids.pdf
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Drugs listed in the Japanese pharmacopeia (17th edition)
Ciprofloxacin [AP-1519]
Voriconazole [AP-1520]
Nucleic acids and related compounds
Allantoin, hypoxanthine and other nucleic acid-related compounds [AP-1497, AP-1518]
Nucleotides: AMP, ADP, ATP [AP-1499]
Sugar nucleotides [AP-1492]
Vitamin D metabolites
Vitamins D2 and D3 [AP-1515]
Vitamins D2 and D3 and their metabolites [AP-1514]
Food components
Soy oligosaccharides: raffinose and stachyose [AP-1487, AP-1495]
Stevia extract: stevioside and rebaudioside A [AP-1500]
Rice bran extract: γ-oryzanol [AP-1508, AP-1509]
Polyphenol oligomers [AP-1510, AP-1511, AP-1512]
Quercetin glycosides [AP-1516]
Nacalai USA provides the highest quality research products necessary for the Life Science research such as Molecular Biology and Proteomics, and also provide Cosmosil brand HPLC columns. Most of the products are manufactured and packaged in the high level of manufacturing facilities in Japan.
