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  • ハッシュタグ「#reference」の検索結果21件

Simultaneous analysis of DL-Amino acids in foods and beverages using a highly sensitive chiral resolution labeling reagent

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Simultaneous analysis of DL-Am...
 
Highlights 
•Our chiral resolution labeling reagent D-FDLDA has high MS sensitivity and separation ability for each DL-amino acid.
•DL-amino acids in foods and beverages were separated and quantified via LC-MS using a C18 column by labeling with D-FDLDA.
•Each DL-amino acid peak was accurately identified using a stable isotope-labeled D-FDLDA (13C6-D-FDLDA).
•Samples labeled with D-FDLDA can be stored for at least 1 week at 4 °C.
 
#19942-74 DL-Amino Acid Labeling Kit is used in this study. 
#reference

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Reference: In-situ Myocardial Regeneration with Tissue-Engineered Cardiac Patch using Spheroid based 3D tissue

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Reference: In-situ Myocardial ...
#reference

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Simultaneous Separation and Identification of All Structural Isomers and Enantiomers of Aminobutyric Acid.

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Simultaneous Separation and Id...
#aminoacid #chiral #drug #hplc #isomer #labeling #lcms #reference

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Separation of Isoleucine Enantiomers and Diastereomers Using an Original Chiral Resolution Labeling Reagent

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Separation of Isoleucine Enanti...
#DL #aminoacid #chiral #drug #isoleucine #peptide #reference

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Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column

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Separation of highly hydrophili...
We published "Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column" in MethodsX, 2023, 10. 102061.

DOI: https://doi.org/10.1016/j.mex.2023.102061

Abstract: 
Highly hydrophilic compounds such as nicotinamide metabolites are very difficult to separate via high-performance liquid chromatography (HPLC) using octadecyl (C18) columns. In general, for the separation of hydrophilic compounds, hydrophilic interaction liquid chromatography (HILIC) columns are used instead of reversed phase chromatography using C18 columns. However, HILIC columns generally obey complex separation mechanisms because ionic interactions are involved in the retention process, which hinders the optimization of the separation conditions. Additionally, the resulting peak shapes are disturbed when large amounts of aqueous samples are injected. This study demonstrates that COSMOSIL PBr columns, in which both hydrophobic and dispersive interactions occur, show high retention for various hydrophilic compounds under similar separation conditions as those used with C18 columns. Specifically, using a COSMOSIL PBr column, 11 nicotinamide metabolites could be separated under simpler conditions than those used previously with C18 columns, affording better peak shape for each compound. The applicability of the method was evaluated using a tomato sample, from which the nicotinamide metabolites were successfully separated. The results show that the COSMOSIL PBr column is a good alternative to the C18 column for a good separation of all the peaks, including impurity peaks.



The following products were used in this study.
COSMOSIL PBr Packed Column
#NMN #hilic #hplc #lcms #nicotinamide #reference

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Separation of amyloid β fragment peptides with racemised and isomerised aspartic acid residues using an original chiral resolution labeling reagent

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Separation of a...
We are pleased to announce that the study using our DL-amino acid labeling kit, made in collaboration with Professor Hideaki Kakeya from Graduate School of Pharmaceutical Sciences, Kyoto University, was selected for inclusion in HOT Articles 2023 by the Royal Society of Chemistry journal Analyst, 2023, Advance Article


The following products were used in this study.
DL-Amino Acid Labeling Kit
#Aminoacid #DL #HPLC #Labeling #Reference #chiral

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Reference: Separation of long-stranded RNAs by RP-HPLC

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Reference: Separation of long-...
#dna #drug #hplc #mrna #reference #vaccine #widepore

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Reference: Separation of nicotinamide metabolites

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Reference: Separation of nicoti...
#application #hplc #metabolite #nicotinamide #reference

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Reference: Scalable human kidney organoid platform

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Reference: Scalable human kid...
Dr. Andrew McMahon's group published "A scalable organoid model of human autosomal dominant polycystic kidney disease for disease mechanism and drug discovery" in Cell Stem Cell on 7 July 2022.

https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(22)00254-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1934590922002545%3Fshowall%3Dtrue

The following products were used in this study.

StemFit Basic02:
https://www.nacalaiusa.com/products/view/351/stemfit-basic-series-by-ajinomoto-ips-es-cell-culture-media

EZSPHERE:
https://www.nacalaiusa.com/products/view/256/ezsphere-trade-for-3d-cell-culture-with-microwells
#reference

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Reference: New amino-acid labeling reagent, (D)FDLDA

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Reference: New amino-acid lab...
"Separation and identification of the DL-forms of short-chain peptides using a new chiral resolution labeling reagent" was published in Anal Bioanal Chem on 06 April 2022.
https://doi.org/10.1007/s00216-022-04048-w


The following products were used in this study.

*** DL-Amino Acid Labeling Kit ***
https://www.nacalaiusa.com/products/view/528/dl-amino-acid-labeling-kit

*** COSMOCORE 2.6C18 packed column ***
https://www.nacalaiusa.com/products/view/235/cosmocore-2-6c-sub-18-sub

*** COSMOCORE 2.6Cholester packed column***
https://www.nacalaiusa.com/products/view/254/cosmocore-2-6cholester

*** COSMOSIL 3PBr packed column ***
https://www.nacalaiusa.com/products/view/238/cosmosil-pbr

*** COSMOSIL CHiRAL 3C packed column ***
https://www.nacalaiusa.com/products/list/71/chiral-separation-columns
#Chiral #HPLC #LCMS #aminoacids #peptides #reference

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