Nacalai USA's Blog

Taurine is a biologically significant molecule with a zwitterionic character, which makes it a difficult analysis target. COSMOSIL HILIC has a unique anion-exchange mechanism which allows strong retention of taurine with good peak shape. It is USP packing type L104, specified on the official monogram for taurine.

>> General information on COSMOSIL HILIC
https://www.nacalaiusa.com/products/view/96/cosmosil-hilic

>> USP revision notice for taurine (PDF file)
https://www.uspnf.com/sites/default/files/usp_pdf/EN/USPNF/revisions/taurine-rb-notice-20190301.pdf

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size.

COSMOSIL Application
https://www.nacalai.co.jp/cosmosil/data/csmosrchtop.cfm?lc=E

Comparison of Retention Behavior between Supercritical Fluid Chromatography and Normal-Phase High-Performance Liquid Chromatography with Various Stationary Phases

Reference:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650800/

COSMOSIL SFC Columns:
https://www.nacalaiusa.com/products/list/63/cosmosil-sfc-column

Due to the lack of retention on ODS columns, oligosaccharides have typically been analyzed on specialty sugar columns. We have successfully analyzed inulins using COSMOSIL Sugar-D, a specialty column for saccharide analysis, and PBr, a unique reversed-phase column for polar and non-polar analytes.

About Inulin
Inulin structure Inulin (G-Fn-F) is a polysaccharide composed of a glucose unit (G) bonded to multiple fructose units (F). It is an indigestible, water-soluble dietary fiber, abundant in the roots of chicory and tubers of Jerusalem artichokes. Recently, oolong tea with inulin has been released as a functional food in Japan on the basis that inulins feed intestinal bacteria by forming short-chain fatty acids and facilitate the peristaltic movement in the bowels. In the literature as well, there are references to inulin's effect on bowel movements.

Click the link below for more information;
https://www.nacalai.co.jp/global/cosmosil/inulin_analysis.html

Data courtesy of Pfizer Inc. La Jolla, California
http://www.nacalaiusa.com/products/view/342/cosmosil-pye

Hydrophilic interaction chromatography (HILIC) is a popular technique to analyze very polar molecules. However, it is often difficult to develop a robust method.

HILIC Columns: Disadvantages
・　Extremely sensitive to sample solvent used resulting in shifting retention time.
・　Small sample loading capacity – polar molecules do not dissolve well in organic solvents.
・　Peak distortion occurs if sample solvents are different than the mobile phase.

PBr Columns: Advantages
・　Retain very polar compounds in reversed-phase.
・　Use simple mobile phase, e.g. 100% aqueous or organic mobile phase.
・　Higher sample loading capacity than HILIC.
・　Robust
COSMOCORE (Core-shell based)
http://www.nacalaiusa.com/products/view/280/cosmocore-2-6pbr
COSMOSIL (Fully porus silica gel based)
http://www.nacalaiusa.com/products/view/238/cosmosil-5pbr

Feb. 22nd Monday 7:00 PM Poster # 53
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Topic: Metabolomics
Separating Vitamin D2 D3, their 25-OH Metabolites and C-3 Epimers
The accuracy of current vitamin D measurements by immunoassays and LCMS has been questioned due to the overlapping LC peaks with identical m/z values epimers and isobars. To solve this problem, we have developed a new HPLC method to achieve baseline separation of vitamin D2/D3, their 25-OH metabolites and C3-epimers in one single run.
Download (PDF)
http://www.nacalai.co.jp/global/cosmosil/pdf/MSACL2016_vitaminD.pdf
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Feb. 22 nd Monday 7:00 PM Poster # 54
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Topic: Small Molecule Analytes
Detecting THC Metabolites and Other Cannabinoids
In the first part, Δ9-THC, 11-hydroxy-Δ9-THC and 11-nor-9-carboxy-Δ9-THC are detected using a simple gradient. In the second part, four cannabinoids, CBD, CBN, Δ9-THC, and Δ8-THC were baseline separated making quantification easy.
Download (PDF)
http://www.nacalai.co.jp/global/cosmosil/pdf/MSACL2016_cannabinoids.pdf
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Not getting enough separation or retention with your C18 column? Watch this tutorial to learn how to improve your analysis.



More information on COSMOSIL Cholester
http://www.nacalaiusa.com/products/view/61/cosmosil-cholester
More information on COSMOSIL PBr
http://www.nacalaiusa.com/products/view/238/cosmosil-5pbr

New applications for drugs listed in the Japanese pharmacopeia, metabolites and foods have been added to our database.

To see each application, click the link below and put an application No. (AP-****) into a box.
http://www.nacalai.co.jp/cosmosil/data/csmosrchtop.cfm?&lc=E

Drugs listed in the Japanese pharmacopeia (17th edition)
Ciprofloxacin [AP-1519]
Voriconazole [AP-1520]

Nucleic acids and related compounds
Allantoin, hypoxanthine and other nucleic acid-related compounds [AP-1497, AP-1518]
Nucleotides: AMP, ADP, ATP [AP-1499]
Sugar nucleotides [AP-1492]

Vitamin D metabolites
Vitamins D2 and D3 [AP-1515]
Vitamins D2 and D3 and their metabolites [AP-1514]

Food components
Soy oligosaccharides: raffinose and stachyose [AP-1487, AP-1495]
Stevia extract: stevioside and rebaudioside A [AP-1500]
Rice bran extract: γ-oryzanol [AP-1508, AP-1509]
Polyphenol oligomers [AP-1510, AP-1511, AP-1512]
Quercetin glycosides [AP-1516]

Active pharmaceutical ingredients
Anti-diabetic drug: acarbose [AP-1486]
Polymyxin antibiotic: colistin [AP-1502]